Lipopolysaccharides increase the risk of colorectal cancer recurrence and metastasis due to the induction of neutrophil extracellular traps after curative resection

Intra-abdominal infection after curative surgery for colorectal cancer is a serious complication associated with an increased risk of recurrence. Lipopolysaccharides (LPS)—an essential component the cell wall Gram-negative bacteria—were found to exert protumorigenic effect by stimulating inflammatory pathology and formation neutrophil extracellular traps (NETs). This study was conducted test whether LPS-induced NETs promotes development metastasis. The clinical characteristics, incidence relapse, serum myeloperoxidase–DNA complexes 40 patients without were analyzed. effects LPS on induction evaluated in mouse model liver toll-like receptor 9 (TLR9)—a DNA receptor—was knocked down assess its mitogen-activated protein kinase pathway activities implicated NETs. Analysis data obtained from these showed significant relation metastasis survival rates. Subsequent vitro experiments revealed level citrullinated-histone H3 LPS-injected mice cancer. In mimic metastatic model, injection enhanced capacity, which then attenuated DNase I. suggested that activated LPS. Injection TLR9-knockdown HCT116 cells mice, followed through LPS, mitigated myeloperoxidase–DNA. finding implied suppressed. These findings shed light mechanism underlying relationship between elevated rate recurrence who underwent infection. involves could be mediated TLR9 signaling pathway.